In vitro propagation of carnation dianthus caryophyllus l. Therefore, the therefore, the present study was designed to develop effective explants sterilization protocol for shoot tip and leaf sheath explants of. In vitro propagation of an ornamental aquatic plant, anubias barterii var. In vitro micropropagation of aloe adigratana reynolds. Section b protocols for in vitro propagation of ornamental plants 27 in vitro production of sweet peas lathyrus odoratus l. In vitro tissue culture techniques show a certain promise with respect to a future increase in production. Micropropagation of an exotic ornamental plant, calathea. Propagation of ornamental trees, shrubs, and woody vines introduction ornamental trees, shrubs, and woody vines are perennial plants. A number of in vitro protocols have been developed for regeneration of gladiolus plantlets by using various explants sources of the plant. Michael kane environmental horticulture department university of florida rapid clonal in vitro propagation of plants from cells, tissues or organs cultured aseptically on defined media contained in culture vessels maintained under controlled conditions of light and temperature micropropagation in vitro propagation. An in vitro propagation protocol was therefore established with the regeneration of 12 shoots per shoottip explant within 8 weeks using murashige and skoog ms medium supplemented with 2.
Under the longterm in vitro process, various factors such as media composition and plant growth regulators may result in variations in regenerated plants 15. Seeds of carissa have short viability and should be sown just after extraction from fruits kumar et al. While ornamental plants are produced mainly for their aesthetic value, the propagation and improvement of quality attributes such as leaf types, flower colour and fragrance, longevity and form, plant shape and architecture, and the creation of novel variation are important economic goals for the expanding ornamental industry. Micropropagation is a powerful tool for largescale propagation of ornamental plants. Lisianthus eustoma grandiflorum is an ornamental plant with beautiful flowers. In addition to the detailed protocols of in vitro shoot initiation, proliferation, root induction and acclimatization, chapters also include detailed. Clonal propagation of cyclamen persicum via somatic embryogenesis traud winkelmann section b. A simple and efficient protocol for the mass propagation. In vitro propagation of these species was established using shoot tip explants. The biology and in vitro propagation of the ornamental aquatic plant, aponogeton ulvaceus. Divided into two sections, section a contains chapters mainly on micropropagation. Explants sterilization protocol for invitro propagation. A protocol has been developed for in vitro plant regeneration from a nodal explant of dracaena sanderiana sander ex mast.
Pdf protocol for in vitromicropropagation of ornamental. Several protocols for the in vitro propagation of this species including nodal segment culture, somatic embryogenesis, and adventitious shoot induction are described in this chapter. Clonal propagation from tissues of ex vitro adult plants may not represent the real variability of species, besides. This study was carried out in the plant tissue culture laboratory, at elzohria botanical garden. The rate of micropropagation and plant length, rooting rate, numbers of root and root length were. Jacas 28 in vitro conservation and cryopreservation of ornamental plants 303 elifaylin ozudogru, alberto previati, and maurizio lambardi. Protocols for micropropagation of selected economically. Clarke, an endemic and rare plant of meghalaya, has been established.
However, literature is rather scanty on in vitro cormel formation and acclimatization of in vitro propagules. In vitro plant regeneration from leaf and petiole explants of begonia rubrovenia meisneri c. Micropropagation of lisianthus eustoma grandiflorum, an ornamental plant. Comprehensive and clear, protocols for in vitro propagation of ornamental plants presents key techniques that will be of great use to floriculturists, researchers, commercial companies. This quality makes it an ideal system for in vitro regeneration experiments. Nana petite sheeja george ea, aneykutty josephaand alphi korathb aschool of marine sciences, cochin university of science and technology, fine arts avenue, kochi682 016, kerala, india bschool of management and entrepreneurship, kerala university of fisheries and ocean. Sakr department of ornamental horticulture, faculty of agriculture, cairo university, giza, egypt abstract. Pdf protocols for in vitro propagation of ornamental plants methods in molecular biology jennie216. Conventional propagation is clearly not suitable to overcome problem and therefore.
Therefore, we explored an efficient protocol for in vitro plant regeneration via shoot induction of m. Increasing demand for ferns means that more knowledge of propagation techniques be developed to guarantee the continuous supply of these plants from commercial ornamental companies. Anoectochilus roxburghii orchidaceae family is an important medicinal and ornamental plant which is in extinct condition because of the overcollected and the loss of its suitable habitats. In vitro propagation of gladiolus plantlets and cormels. A successful protocol was established for micropropagation in two selected varieties of exotic ornamental plants, calathea crotalifera. Micropropagation through in vitro culture techniques is an ideal approach for largescale propagation of ornamental potted plants, including those having value in the foliage and flowering plants. In vitro propagation of five alocasia species scielo. In this elaboration, effective methods of in vitro propagation of a paulownia tomentosa.
In vitro propagation of matthiola incana brassicaceaean. Plant in vitro 24 time, through culturing explants in controlled laboratory environmental condition. Pdf protocols for in vitro propagation of ornamental plants. Pdf download protocols for in vitro propagation of ornamental plants methods in molecular. Therefore, the genetic uniformity assessment of regenerated plants is of great importance. In vitro propagation of an ornamental aquatic plant. In vitro propagation protocol for dieffenbachia amoena. Gesneriaceae is a popular ornamental pot plant that is easy to culture ex vitro and in vitro relative to other herbaceous ornamentals. This study aimed at developing a suitable and reproducible protocol for in vitro micropropagation of aloe adigratana reynolds using explants from offshoots with the help of the most commonly used plant growth regulators pgrs. M 2,4dichlorophenoxyacetic acid 2,4d followed by 46.
Divided into two sections, section a contains chapters mainly on. While ornamental plants are produced mainly for their aesthetic value, the propagation and improvement of quality attributes such as leaf types, flower colour and fragrance, longevity and form, plant. An efficient micropropagation protocol for an endangered. In popular medicine it is used in infusions such as. Protocol for in vitromicropropagation of ornamental pineapple. In conclusion, an in vitro propagation protocol discussed here can be applied for largescale production of plants of b. The plant produces offshoots from tubercles very rarely, and the seedlings exhibit slow growth and susceptibility to damping off. Micropropagation is an effective tools for propagation of ornamental plants in large scale. In vitro propagation of matthiola incana brassicaceaean ornamental plant.
Micro propagation technique has been employed in the propagation of tree species, woody species, immature embryos, hypocotyl, young leaves, cotyledons, ovary, ovule and other juvenile tissues as good source of tissue for the propagation of several woody plants. In vitro regeneration and propagation of enset highly inhibited due to microbial contamination 16,17. A few ornamental shrubs and vines are used as annuals and planted in landscapes for one season in hardiness zones where they will not survive winter temperatures. Jain sm, ochatt sj 2010 protocols for in vitro propagation. Thus, the aim of this research is to establish a simple and efficient micropropagation pro tocol with further development third subculture for plant tissue culture of vanilla for large scale production. Pdf while ornamental plants are produced mainly for their aesthetic value, the propagation and improvement of quality attributes such as leaf. In vitro propagation of medicinal and ornamental plant. Divided into two sections, section a contains chapters mainly on micropropagation of cut and pot flowers with detailed protocols involving in vitro cultureexplants, medium preparation, detailed medium table, shoot initiation and proliferation, root induction, in vitro plant.
Micropropagation is the rapid vegetative propagation of plants under in vitro conditions of high light intensity, controlled temperature and a defined nutrient medium. Divided into two sections, the first section contains chapters mainly on micropropagation of cut and pot flowers with detailed protocols involving in vitro culture. The effects of different sterilization techniques, explant type, and the combination and concentration of plant growth regulators on shoots induction were studied. Actually, in vitro culture techniques performance a prominent role in the multiplication and maintenance of commercially propagated ornamental plant species, and. Elephant tusk cactus coryphantha elephantidens lem. Due to low percentage of seed viability and destructive. Here, a protocol was developed for flowering and high frequency in vitro micropropagation of e.
Protocols for in vitro propagation of ornamental plants. Read in vitro mutagenesis protocols methods in molecular biology ebook free. Micropropagation is an effective tools for propagation of. In vitro production of sweet peas lathyrus odoratus l. An efficient protocol was established for in vitro propagation of c. Micropropagation of lisianthus eustoma grandiflorum plant omics. The optimized conservation protocols also allow an immediate. Propagation of ornamental trees, shrubs, and woody vines.
Effects of different media on micropropagation and rooting of myrtle. Protocols for in vitro propagation of ornamental plants nhbs. Micropropagation of two species of micranthocereus. Studies on micropropagation of ten weeks and lisianthus is relatively low, thus, here we present a reliable method for in vitro propagation of these two ornamental plants. Lisianthus, in vitro propagation, kinetin, naphthalene acetic acid.
The ex vitro rooting along with simultaneous hardening made the protocol more economical and shortened the in vitro regeneration protocol of b. A highly efficient in vitro propagation protocol for. Pdf in vitro propagation of begonia roubrovenia a rare. Nodal explant showed high callus induction potentiality on ms medium supplemented with 6.
The ex plants were collected from the well developed true to type mother plants. In vitro propagation protocol for dieffenbachia amoena tropic snow plant weaam r. The first three sections section a, b and c contain 29 micropropagation protocols of selected fruit and nut species, indoor and outdoor ornamental plants, cut flowers, and vegetables. Preliminary entry 2244 methods in molecular biology. This chapter summarizes the application of the most commonly used in vitro propagation techniques for trees, shrubs and herbaceous species that can be implemented on a. Protocols for micropropagation of ornamental plants. The newer tissue material obtained through r dna technology or haploid culture or somatic hybridization can be the source of tissue material for micro propagation, as it is the easiest method for obtaining the multiple propagules. In protocols for in vitro propagation of ornamental plants, leading researchers in the field compile stepwise protocols for rapid plant multiplication and in vitro storage of major commercially viable ornamental plants. Micro propagation is one of the finest ways of plant multiplication by in vitro technique of plant tissue culture. Pdf micropropagation of ornamental plantscut flowers. Micropropagation through an in vitro culture technique is a reliable biotechnological tool that holds a better scope and future for the propagation of numerous economically prominent ornamentals, including those having value in the foliage and flowering plants industry.
Slow growth and high demand in the cactus industry lead to finding an alternate fast propagation method. Micropropagation of lisianthus eustoma grandiflorum, an. The biology and in vitro propagation of the ornamental. The percentage seed germination varied with the composition of the nutrient media and was highest in fullstrength ms basal medium. The shoot tips explants from lisianthus were cultured on ms medium supplemented with concentrations of 0, 0. The biology and in vitro propagation of the ornamental aquatic plant, aponogeton ulvaceus melissa yit yee kam, li chin chai and chiew foan chin abstract aponogeton ulvaceus baker aponogetonaceae is a commercially important ornamental aquatic plant species with traditional medicinal uses. Pdf in vitro micropropagation of the ornamental plant. Simplified method of in vitro propagation of spathiphyllum floribundum l petite for the mass propagation has been developed. In vitro conservation of ornamental plants 1 scielo. In vitro micropropagation of dracaena sanderiana sander ex. Comprehensive and clear, protocols for in vitro propagation of ornamental plants presents key techniques that will be of great use to floriculturists, researchers, commercial companies, biotechnologists, and students dealing with ornamental plants. In vitro flowering in micro propagation techniques can shorten juvenile period. The technique has been applied to a substantial number of commercial vegetatively propagated plant species.
Several protocols for the in vitro propagation of this species including nodal segment culture, somatic embryogenesis, and adventitious shoot induction are described. Methods in molecular biology methods and protocols, vol 589. In vitro propagation of matthiola incana brassicaceae. Multiple shoots were induced on murashige and skoog ms medium supplemented with. Protocols for in vitro propagation of ornamental plants springerlink. Noelle dorion, hatem ben jouira, anthony gallard, anber hassanein, mazen nassour, agnes grapin.